BBD-RSM을 사용한 길경(桔梗) 중의 platycodin D 계열 성분의 추출조건 최적화

초록

This study investigated the effects of extraction conditions on platycodin D, D2, and D3 from the roots of Platycodon grandiflorus, and predicted optimal extraction conditions using Box-Behnken design-based response surface methodology. Extraction time, ethanol concentration, liquid-to-solid ratio, and extraction temperature were selected as independent variables. Twenty-seven experiments were conducted, and the contents of platycodin D, D2, and D3 were quantified by UPLC-ESI-QTOF/MS using EIC peak areas. The quadratic regression models were statistically significant, with coefficients of determination (R²) of 0.8890, 0.9040, and 0.8960, respectively. Platycodin D and D2 were significantly affected by extraction time, ethanol concentration, and extraction temperature, whereas ethanol concentration had the greatest effect on platycodin D3. The predicted optimal conditions were 180.0 min, 0%, 10.0 v/w, and 57.7°C for platycodin D; 60.0 min, 0%, 27.0 v/w, and 20.0°C for platycodin D2; and 112.4 min, 39.3%, 30.0 v/w, and 48.7°C for platycodin D3. The differences in the extraction behavior of three major saponins with the same aglycone under the extraction conditions may be associated with variations in their glycoside structures, suggesting structure-dependent extraction behavior among structurally related saponins. These results provide useful information for selecting extraction conditions according to the target saponin.

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