Antioxidant and anti-inflammatory effects of the extract from rhizomes of Belamcanda chinensis (L.) DC. on human keratinocyte HaCaT cells

초록

Chronic oxidative stress and inflammation play critical roles in the development of various skin disorders, and plant-derived polyphenols have attracted considerable attention as potential therapeutic agents due to their antioxidant and anti-inflammatory properties. In the present study, the antioxidant and anti-inflammatory activities of Belamcanda chinensis (L.) DC. (BC) rhizome extract on human skin keratinocytes were investigated. The chemical constituents of the BC extract were analyzed using high-performance liquid chromatography coupled with mass spectrometry, which identified five major polyphenolic compounds. The antioxidant capacity of the extract was evaluated using ultrafiltration-liquid chromatography targeting 2,2-diphenyl-1-picrylhydrazyl radicals. In lipopolysaccharide-stimulated HaCaT keratinocytes, BC extract significantly reduced the expression of inflammatory mediators, including cyclooxygenase-2 and inducible nitric oxide synthase and modulated the signal transducers and activators of transcription 3/nuclear factor-kappa B (NF-kappa B) signaling pathway. Furthermore, molecular docking analysis revealed strong binding affinities between NF-kappa B and the five identified polyphenols (tectoridin, iridin, genistein, tectorigenin and irisflorentin), supporting their potential biological relevance. In conclusion, the present findings demonstrated that BC extract exerts notable antioxidant and anti-inflammatory effects through polyphenol-mediated modulation of NF-kappa B signaling, providing a scientific basis for its potential use in anti-inflammatory drug development.

키워드