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- Kim, Ajung;
- Bae, Yeonju;
- Gadhe, Changdev G.;
- Jung, Hyun-Gug;
- Yang, Esther;
- ... Kang, Dawon;
- 외 9명
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0초록
TWIK-1 belongs to the two-pore domain K+ (K2P) channel family, which plays an essential role in the background K+ conductance of cells. Despite the development of exon 2-deleted Twik-1 knockout (KO) mice, the physiological role of TWIK-1 has remained largely unknown. Here, we observed that the exon 2-deleted Twik-1 KO mice expressed an internally deleted TWIK-1 (TWIK-1 ΔEx2) protein, which unexpectedly acts as a functional K+ channel. The Twik-1 nKO mice in which exon 1 was targeted using the CRISPR-Cas9 technique provides strong evidence that TWIK-1 mediates K+ currents that are responsible for the background passive conductance in astrocytes. Deficiency of TWIK-1-mediated astrocytic passive conductance increased susceptibility to kainic acid-induced seizures. This study paves the way for functional studies on TWIK-1-mediated astrocytic passive conductance. In addition, the exon 1-targeted Twik-1 KO mice would help elucidate the physiological roles of TWIK-1. © 2024 The Author(s)
키워드
- 제목
- Mice deficient in TWIK-1 are more susceptible to kainic acid-induced seizures
- 저자
- Kim, Ajung; Bae, Yeonju; Gadhe, Changdev G.; Jung, Hyun-Gug; Yang, Esther; Kim, Hyun; Lee, Jaekwang; Shim, Chanseob; Sung, Young Hoon; Noh, Junyeol; Kim, Eun-Jin; Kang, Dawon; Pae, Ae Nim; Hwang, Eun Mi; Park, Jae-Yong
- 발행일
- 2025-01
- 유형
- Article
- 저널명
- iScience
- 권
- 28
- 호
- 1