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Molecular characterization of the GmAMS1 gene encoding beta-amyrin synthase in soybean plants
- Chung, E.;
- Cho, Ch.-W.;
- Kim, K.-Y.;
- Chung, J.;
- Kim, J.-I.;
- 외 3명
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10초록
Suppression subtractive hybridization was used to isolate wound-induced cDNAs from soybean plants. One of wound-induced cDNA, gmwi33 designated GmAMS1, showed a high homology with genes encoding beta-amyrin synthase. GmAMS1 protein showed 89% identity with licorice GgbAS1. In five-day-old dark-grown seedlings, RNA expression of GmAMS1 was strongly induced by light, weakly induced by methyl jasmonate and low temperature, and was not induced by elicitor or UV-B treatment. Genomic blot and FISH analyses confirmed that the soybean genome has two copies of the GmAMS1 gene. To our knowledge, this is the first report on beta-amyrin synthase from soybean.
키워드
Glycine max; wounding; stress; FISH; FUNCTIONAL EXPRESSION; OXIDOSQUALENE CYCLASE; METHYL JASMONATE; CLONING; SAPONIN; ACID; BIOSYNTHESIS; TRITERPENE; SQUALENE; YEAST
- 제목
- Molecular characterization of the GmAMS1 gene encoding beta-amyrin synthase in soybean plants
- 저자
- Chung, E.; Cho, Ch.-W.; Kim, K.-Y.; Chung, J.; Kim, J.-I.; Chung, Y.-S.; Fukui, K.; Lee, J.-H.
- 발행일
- 2007-07
- 유형
- Article
- 권
- 54
- 호
- 4
- 페이지
- 518 ~ 523