트레할로스 연료전지용 이효소-전자전달체 고정화 전극의 제작 및 전기화학적 특성 분석

초록

In this study, a trehalose enzymatic fuel cell was fabricated and evaluated using carbon paper electrodes modified with single-walled carbon nanotubes (SWCNTs), consisting of a glucose oxidase (GOx)-trehalase (TREH)immobilized anode and a bilirubin oxidase (BOD)-immobilized cathode. In particular, ferrocene methanol (FcMeOH) and 2,2 '-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) were co-immobilized with the enzymes on the anode and cathode, respectively. SEM-EDS analysis confirmed the successful introduction of SWCNTs, enzyme, and electron transfer mediator onto the electrode surfaces. Cyclic voltammetric analysis revealed a distinct oxidation peak of FcMeOH near 0.2 V, and the SWCNT-modified electrode (CP-SWCNT) exhibited a higher oxidation current than the bare CP. In addition, biochemical analysis demonstrated that GOx immobilized on the CP-SWCNT electrode showed higher enzymatic activity. EIS analysis indicated that the introduction of SWCNTs reduced the charge transfer resistance and improved the electron transfer properties of the electrode. The GOx-TREH bienzyme electrode generated a high oxidation current in the presence of trehalose. In addition, the BOD-based cathode exhibited a high reduction current under oxygen-saturated conditions. The fabricated trehalose enzymatic fuel cell exhibited an open-circuit voltage of approximately 0.6 V and a maximum power density of 11.7 +/- 0.9 mu W/cm(2), demonstrating that the SWCNT-based electrode modification and enzyme/electron transfer mediator immobilization strategy were effective for improving enzymatic fuel cell performance.

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