The co-repressor protein HOS15 negatively regulates ethylene signaling via degradation of EIN3 during arabidopsis apical hook formation

초록

Ethylene signaling is essential for apical hook formation during early seedling development in darkness, protecting cotyledons and shoot apical meristem as seedlings emerge through soil. ETHYLENE-INSENSITIVE 3 (EIN3), a central transcription factor in this pathway, activates ethylene-responsive genes such as HOOKLESS 1 (HLS1) to promote hook formation. EIN3 is rapidly stabilized in response to ethylene but is otherwise targeted for degradation by the F-box proteins EIN3-BINDING F BOX PROTEIN 1 (EBF1) and EBF2. Here, we demonstrate that HIGH EXPRESSION OF OSMOTICALLY RESPONSIVE GENE 15 (HOS15), a substrate receptor for both SCF/ CUL1 and CUL4-DDB1 E3 ubiquitin ligase complexes, functions as a negative regulator of EIN3 protein stability. Loss-of-function mutation of HOS15 (hos15-2) resulted in enhanced apical hook curvature upon treatment with the ethylene precursor 1-aminocyclopropane-1-carboxylic acid (ACC). In these mutants, ethylene-induced HLS1 transcript levels were significantly higher than those in wild-type plants, indicating that HOS15 suppresses ethylene-mediated hook formation. HOS15 interacts with EIN3 in planta and promotes its destabilization. Furthermore, the hos15-2 ein3-1 double mutant exhibited reduced hook curvature and lower expression of HLS1 and ETHYLENE RESPONSIVE FACTOR 1 (ERF1) compared with hos15-2 alone, indicating that EIN3 functions downstream of HOS15. These findings establish HOS15 as a key modulator of ethylene signaling, fine-tuning EIN3 protein stability during apical hook development in Arabidopsis thaliana.

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