Isolation and identification of compound from dropwort (Oenanthe javanica) with protective potential against oxidative stress in HepG2 cells

Choi, Heejung; You, Yanghee; Hwang, Kwontack; Lee, Jeongmin; Chun, Jiyeon; Chung, Jin Woong; Shim, Sangin; Park, Chang-Soo; Jun, Woojin

doi:10.1007/s10068-011-0240-1

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Isolation and identification of compound from dropwort (Oenanthe javanica) with protective potential against oxidative stress in HepG2 cells

Choi, Heejung;
You, Yanghee;
Hwang, Kwontack;
Lee, Jeongmin;
Chun, Jiyeon;
... Shim, Sangin;
외 3명

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11

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15

초록

The protective effect of dropwort (Oenanthe javanica) was investigated in HepG2 cells under oxidative stress, and its active compound was identified. Compared to H(2)O(2)-alone treated cells, a drastic increase (35%) in protective activity was observed in the cells pretreated with 80% ethanol extract (OJE) of dropwort, suggesting that OJE possessed a potent hepatoprotectant. After a sequential procedure consisting of solvent-partitioning chromatography, TLC, and HPLC was conducted, the compound with proven hepatoprotective activity from OJE was isolated and identified as caffeic acid. Results indicated that caffeic acid in dropwort contributed to this herb's protective profile against oxidative stress-induced liver damage.

키워드

dropwort (Oenanthe javanica); HepG2 cell; caffeic acid; oxidative stress; ANTIOXIDANT ACTIVITY; JAPONICA LEAVES; NITRIC-OXIDE; CAFFEIC ACID

제목: Isolation and identification of compound from dropwort (Oenanthe javanica) with protective potential against oxidative stress in HepG2 cells

저자: Choi, Heejung; You, Yanghee; Hwang, Kwontack; Lee, Jeongmin; Chun, Jiyeon; Chung, Jin Woong; Shim, Sangin; Park, Chang-Soo; Jun, Woojin

DOI: 10.1007/s10068-011-0240-1

발행일: 2011-12

유형: Article

저널명: Food Science and Biotechnology

권: 20

호: 6

페이지: 1743 ~ 1746

언어: ENG

출판사: SPRINGER

발행국가: 미국

분량: 4 페이지

ISSN: E 2092-6456
P 1226-7708