콩에서 Rs2 (rs2) 유전자 마커에 의한 stachyose 함량 분석

초록

Soybeans contain the three major nutrients－protein, fat, and carbohydrates－as well as various functional components and are widely used for human and livestock consumption. However, the stachyose component of mature soybean seeds is a nondigestible sugar; thus soybean varieties with low stachyose content must be developed. A study was conducted to evaluate the validity of marker-assisted selection (MAS) using a DNA marker based on the Rs2 (rs2) gene sequence in the breeding of soybean lines with low stachyose content. The marker analysis results showed that all varieties, including “Daewon”, “Jinpum#2”, “Miso”, “Pungsannamul”, “Cheongja#3”, and “Seoritae” had the Rs2Rs2 genotype, and all three breeding lines were found to have the rs2rs2 genotype. The stachyose content (g/kg) analyzed via HPLC showed variation between replicates and genotypes, ranging from 9.1 to 14.0 g/kg in the six varieties and from 2.4 to 3.7 g/kg in the three breeding lines. The range and average stachyose content in the Rs2Rs2 genotype were 9.1–14.0 g/kg and 11.56±1.49 g/kg, respectively, while in the rs2rs2 genotype, they were 2.4–3.7 g/kg and 3.01±0.37 g/kg, respectively, which were significantly lower. In breeding soybean lines with low stachyose content, the RS2 (rs2) gene MAS technique could be highly effective for selecting individuals with the rs2rs2 genotype.

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