LPS에 의한 염증 유도 동물모델에서 유채 새싹 추출물의 보호 효과

초록

This study evaluated the anti-inflammatory effects and its underlying mechanisms of Brassica napus L. sprouts (BN) extract in a lipopolysaccharide (LPS)-induced inflammation mouse model. Mice were randomly assigned to five groups: Control, LPS, BN100 (100 mg/kg/day), BN400 (400 mg/kg/day), and DEX (dexamethasone, 2 mg/kg/day). The mice were orally administered the indicated treatments for 7 consecutive days, followed by intraperitoneal injection of LPS at a dose of 10 mg/kg to induce acute systemic inflammation. Treatment of LPS significantly increased serum aspartate aminotransferase and alanine aminotransferase activities, as well as the levels of the pro-inflammatory cytokines tumor necrosis factor-α, interleukin (IL)-1β, and IL-6. In addition, hepatic lipid peroxidation, nitric oxide, and reactive oxygen species levels were markedly elevated in LPS-treated mice, accompanied by hepatic histopathological damage. Furthermore, LPS-treated mice exhibited increased expression of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and pro-inflammatory cytokines via activation of nuclear factor-kappa B (NF-κB) signaling in liver. In contrast, administration of BN extract significantly attenuated these alterations by improving serum biochemical markers, reducing oxidative stress, and alleviating hepatic histopathological damage. Moreover, BN administration downregulated the expression of p-NF-κB, iNOS, COX-2, and pro-inflammatory cytokines. These findings suggest that BN attenuates LPS-induced inflammation and liver injury through regulation of the NF-κB signaling pathway and may be a promising functional food ingredient for the prevention of inflammation-associated liver diseases.

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