Antioxidant activity of edible sprouts and phytosterol contents by HPLC/UV analysis
  • Kim, Juree
  • Kim, Ji Hyun
  • Bang, Se In
  • Shin, Hanna
  • Cho, Eun Ju
  • 외 1명
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초록

This study evaluated the in vitro radical scavenging activities of edible tree sprouts, particularly those of the 2,2-diphenyl-1-picrylhydrazyl radical (DPPH), hydroxyl radical, and singlet oxide radical, to assess their antioxidant activities. Additionally, stigmasterol (ST) and beta-sitosterol (BS) were analyzed using HPLC/UV. The edible sprouts of Eleutherococcus senticosus (ESC) and Morus alba (MAB) exhibited the highest DPPH scavenging activity among other edible sprouts. A reverse-phase column was used in an isocratic elution system, after which UV detection was performed at 210 nm. ST and BS analyses indicated that ESC sprouts contained the highest amounts of ST (9.99 mg center dot g(- 1) extract), whereas MAB sprouts contained the highest concentrations of BS (14.69 mg center dot g(- 1) extract). In conclusion, the highest antioxidant activity was observed in the edible sprouts with the highest phytosterol content. Therefore, our findings provide a theoretical basis for the development of plant-based functional foods or supplements with antioxidant properties.

키워드

Antioxidant activityEdible sproutsHPLCUVPhytosterolQuantitative analysisBETA-SITOSTEROLOXIDATIVE STRESSSUBSTANCESFLAVONOIDSTOXICOLOGYEXTRACTSSTEROLSLEAVESOIL
제목
Antioxidant activity of edible sprouts and phytosterol contents by HPLC/UV analysis
저자
Kim, JureeKim, Ji HyunBang, Se InShin, HannaCho, Eun JuLee, Sanghyun
DOI
10.1007/s13580-022-00434-6
발행일
2022-10
유형
Article
저널명
Horticulture, Environment, and Biotechnology
63
5
페이지
769 ~ 778