Elucidating the role of rice thioredoxin-m in mitigating copper toxicity

초록

The pervasive accumulation of elevated heavy metal concentrations in the environment exerts significant selective pressures, driving the progressive evolution of a comprehensive suite of adaptive strategies. These include both physiological tolerance and biochemical detoxification mechanisms. Our research identified a novel role of a rice-specific m-type thioredoxin (OsTrx-m) in conferring tolerance specifically to copper-induced heavy metal stress. Upon exposure to CuSO4, recombinant OsTrx-m underwent a rapid functional transformation from a disulfide reductase to a holdase chaperone, accompanied by a concurrent alteration in protein conformation from a monomeric form to oligomeric complexes. This transition was highly selective for Cu2+ and did not occur with other metal cations, such as Fe2+, Zn2+, Cd2+, and Ni2+. The Cu2+-induced structural and functional transitions of OsTrx-m were reversibly mitigated by adding EDTA, a metal chelator. Comparative analysis of OsTrx-m with Arabidopsis m-type thioredoxins (AtTrx-m1, -m2, and -m3) revealed that this Cu2+-responsive property is a distinctive characteristic of OsTrx-m. Consequently, ectopic expression of OsTrx-m, but not AtTrx-m1, in Arabidopsis (OsTrx-mOE/Col-0 and AtTrx-m1OE/Col-0, respectively) conferred robust tolerance to copper-induced toxicity, as evidenced by enhanced root growth and fresh weight.

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