Deconica coprophila F3-107 균주의 항균 활성 특성

초록

This study aimed to screen for the antimicrobial substances produced by Basidiomycota. The antimicrobial activities of 25 basidiomycete strains were evaluated against Gram-positive bacteria, Gram-negative bacteria, and yeasts. Among the tested strains, Coprinellus radians F3-4, Postia caesia F3-58, and Deconica coprophila F3-107 exhibited strong antimicrobial activity. Peptide-related analysis of the antimicrobial substances indicated that most of the active compounds were secondary metabolites rather than peptide-based compounds. Minimal inhibitory concentration 50 (MIC50) assays were conducted to compare the antimicrobial potency of the selected strains. Among them, D. coprophila F3-107 showed the strongest antimicrobial activity and was therefore selected for further purification. Antimicrobial substances from the cultural supernatant were partially purified through ethanol fractionation, solvent partitioning, and Sep-Pak column chromatography. The ethanol fractionation revealed that most of the antimicrobial activity remained in the supernatant, with the highest activity detected in the supernatant of 50 % ethanol fraction. Chloroform partitioning of this fraction demonstrated that the aqueous layer exhibited the greatest antimicrobial activity. Subsequent purification of the active fraction using a Sep-Pak column showed that the strongest activity was recovered in the 20 %‒30 % acetonitrile eluates. These findings suggest that the major antimicrobial substances produced by D. coprophila F3-107 possessed moderate hydrophobicity, while some of the strongly hydrophilic compounds may have also contribute to the observed activity. Overall, D. coprophila F3-107 was identified as the most promising antimicrobial-producing strain among the Basidiomycota tested, and its antimicrobial activity appeared to be mainly associated with the secondary metabolites that had partially hydrophobic characteristics.

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