Low Density Lipoprotein (LDL)-Antioxidant Flavonoids from Roots of Sophora flavescens

초록

Oxidation of low density lipoprotein (LDL) is strongly implicated as a key process in the onset of atherosclerosis. In this study, nine alkylated (C-10-C-5) flavonoids from Sophora flavescens were examined for their inhibitory effects on copper-induced LDL oxidation. Of the flavonoids tested, sophoraflavanone G (1), kurarinone (2), kurarinol (3), norkurarinol (4), and kuraridin (9) inhibited the generation of thiobarbituric acid reactive substances (TBARS) with IC(50)s of 7.9, 14.5, 22.0, 26.9, and 17.5 mu M, respectively. The most potent inhibitor, compound 1, also demonstrated significant activities in complementary in vitro investigations, such as lag time (130 min at 5 mu M), relative electrophoretic mobility (REM) of ox-LDL (80% inhibition at 20 mu M), and fragmentation of apoB-100 (inhibition of 71% at 20 mu M). Analysis of the structures of these compounds reveals that a resorcinol moiety in the B-ring is strongly correlated with protection of LDL-oxidation.

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