Establishment of withdrawal time and residue analysis in edible tissues of pigs following oral administration of ivermectin

초록

This study was conducted to re-establish the withdrawal time (WT) for ivermectin (IVM) in pigs as part of the introduction of the positive list system (PLS) program. Forty-two healthy pigs were orally administered IVM at doses of 2.4 mg/kg feed (IVM-1, n = 20) and 4.8 mg/kg feed (IVM-2, n = 20) once daily for 7 days. After treatment, tissue samples were collected from four pigs at 1, 3, 5, 7, and 14 days post-administration. Based on a previously established analytical method, residual IVM concentrations in pig tissues were determined using LC-MS/MS. In the IVM-1 group, IVM levels in muscle, liver, kidney, and fat were below the limit of quantification (LOQ) on days 7, 7, 7, and 14 after the final administration, respectively. In the IVM-2 group, IVM levels in muscle, liver, kidney, and fat were below the LOQ on days 7, 14, 7, and 14 after the final administration, respectively. According to the European Medicines Agency guideline on the determination of withdrawal times, the WTs for IVM-1 and IVM-2 in edible pig tissues were established as 8 and 11 days, respectively. In conclusion, the estimated WT of IVM in swine edible tissues was shorter than the currently recommended WT of 14 days for IVM.

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