상세 보기
- Wang, Yan;
- Zhou, Shenting;
- Li, Runyi;
- Liu, Qian;
- Shao, Xixi;
- ... Kang, Min-Kyoung;
- ... Kim, Seon-Won;
- 외 3명
WEB OF SCIENCE
15SCOPUS
15초록
Mevalonate is an important platform compound for the biosynthesis of isoprenoids. It can be synthesized from acetyl-CoA in the presence of nicotinamide adenine dinucleotide phosphate (NADPH) by the introduced mvaES operon in Escherichia coli. The influences of E. coli hosts, acetyl-CoA supply, and NADPH availability were assessed and engineered to improve the production titer and yield of mevalonate from glycerol. As a result, E. coli DH5 alpha was found to be the best host with high specific capability and titer of mevalonate from glycerol. Through the engineering of phosphoketolase-phosphotransacetylase (xPK-PTA) bypass and NADPH availability, a final titer of 7.21 g/L with a specific capability of 1.36 g/g dry cell weight was gained in flask culture. Our work could offer new information to metabolically engineer the mevalonate pathway for the efficient production of isoprenoids.
키워드
- 제목
- Reassessing acetyl-CoA supply and NADPH availability for mevalonate biosynthesis from glycerol in Escherichia coli
- 저자
- Wang, Yan; Zhou, Shenting; Li, Runyi; Liu, Qian; Shao, Xixi; Zhu, Liyan; Kang, Min-Kyoung; Wei, Gongyuan; Kim, Seon-Won; Wang, Chonglong
- 발행일
- 2022-10
- 유형
- Article
- 권
- 119
- 호
- 10
- 페이지
- 2868 ~ 2877