Macromolecular Docking Simulation to Identify Binding Site of FGB1 for Antifungal Compounds
  • Soundararajan, Prabhakaran
  • Sakkiah, Sugunadevi
  • Sivanesan, Iyyakkannu
  • Lee, Keun Woo
  • Jeong, Byoung Ryong
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초록

Fusarium oxysporum, an important pathogen that mainly causes vascular or fusarium wilt disease which leads to economic loss. Disruption of gene encoding a heterotrimeric G-protein-beta-subunit (FGB1), led to decreased intracellular cAMP levels, reduced pathogenicity, colony morphology, and germination. The plant defense protein, Nicotiana alata defensin (NaD1) displays potent antifungal activity against a variety of agronomically important filamentous fungi. In this paper, we performed a molecular modeling and docking studies to find vital amino acids which can interact with various anti fungal compounds using Discovery Studio v2.5 and GRAMMX, respectively. The docking results from FGB1-NaD1 and FGB1-antifungal complexes, revealed the vital amino acids such as His64, Trp65, Ser194, Leu195, Gln237, Phe238, Val324 and Asn326, and suggested that the anidulafungin is a the good antifungal compound. The predicted interaction can greatly assist in understanding structural insights for studying the pathogen and host-component interactions.

키워드

NaD1FGB1Homology modelingMolecular dockingAntifungal compoundsPROTEIN-LIGAND INTERACTIONSFUSARIUM-OXYSPORUMMOLECULAR DOCKINGSCORING FUNCTIONINFECTIONPATHOGENPROGRAMDESIGNENERGYPCR
제목
Macromolecular Docking Simulation to Identify Binding Site of FGB1 for Antifungal Compounds
저자
Soundararajan, PrabhakaranSakkiah, SugunadeviSivanesan, IyyakkannuLee, Keun WooJeong, Byoung Ryong
DOI
10.5012/bkcs.2011.32.10.3675
발행일
2011-10-20
유형
Article
저널명
Bulletin of the Korean Chemical Society
32
10
페이지
3675 ~ 3681