야생 돌나물 흰비단병 발생 보고

초록

In 2024, sclerotium rot occurred in the wild dolnamul (Sedum sarmentosum) on a red bean field hill in Baekjeon-myeon, Hamyang-gun, Gyeongsangnam-do, South Korea. Symptoms of the disease include the lower part of the dolnamul appearing water-soaked, rotting and, gradually withering, until the entire plant dries up and dies. White silky molds appeared on the lesions and the soil surface of the stems in contact with the ground, and many small, brown, round sclerotia were formed. The mycelia were white and silky and grew well on potato agar medium, and many small, brown, round sclerotia were formed. The size of the sclerotia was 1-3㎜, and the width of the mycelia was 4-9㎛. The dolnamul isolate was further identified by analyzing the ribosomal transcribed spacers (ITS) region, amplifying, and sequencing. The results of the phylogenetic analysis confirmed that the dolnamul isolate belongs to the Agroathelia rolfsii taxon. The diagnostic PCR using species-specific primers generated species-specific PCR amplicons. Based on these results, the dolnamul isolate was identified as A. rolfsii. To fulfill Koch's postulates, fungus isolated from dolnmul was artificially infected, and 15 days after inoculation, the same symptoms as those found in the field developed, and re-isolation was performed. This study is the first report of sclerotium rot of dolnamul caused by A. rolfsii, and it is considered to have high value as data for the occurrence and control of sclerotium rot in the future.

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