고염조건에서 형질전환 애기장대의 내성 분석연구

초록

Among the various environmental stresses of plants, high salinity is a representative stress factor that reduces crop production. In the high salt condition, the ionic balance is destroyed, dehydration is induced, the amount of photosynthesis decreases, and the production of reactive oxygen species (ROS) increases in the plant body, thereby plants showed reducing growth. ROS are caused by various stresses, and due to their high responsiveness, they induce cell destruction and death when present in high concentrations. Plants have developed various antioxidant defense system as defense mechanisms. Peroxidase is a representative antioxidant enzyme, and acts as an antioxidant defense against plants by reducing H2O2, a type of free radical species. However, in contrast, there are peroxidase with a catalytic mechanism that increases H2O2, among plant peroxidase, and the produced H2O2, acts as a signal molecule and participates in defense reactions. Among the peroxidase having this mechanism, swpa4 peroxidase gene isolated from sweetpotato, has been reported to be involved in defense mechanisms under various stress conditions through prior studies. The purpose of this study was to determine whether swpa4 is involved in plant resistance under high salt conditions. It was confirmed whether swpa4 is involved in resistance to high salt conditions through analysis of chlorophyll and carotenoid levels in the swpa4 overexpressing transformed Arabidopsis according to various salt concentration conditions. Through the results of this study, it was found that swpa4 was involved in resistance to chlorophyll and carotenoid damage during high salt treatment during first experiment, and during second experiment, chlorophyll and carotenoid of Arabidopsis in the second experiment. No significant results were obtained that were involved in resistance to damage. The first experiment can be said to be more significant because it has fewer errors than the second. Therefore, it was found that swpa4 was involved in resistance to chlorophyll and carotenoid damage when salt concentration was treated. In the second experiment, an uneven rosette leaf was used, and since the expression level of swpa4 in the swpa4-OX lines was not confirmed, additional study is needed to compensate for this when swpa4 is involved in resistance to chlorophyll and carotenoid damage in salt concentration treatment.