Response Surface Methodology Optimization on Ultrasonic-Assisted Extraction of Chicory (Cichorium intybus L.) and Their UPLC-ESI-QTOF-MS Analysis, Antioxidant and Hepatoprotective Activity
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초록

Chicory (Cichorium intybus L.) roots are commonly roasted and ground to produce a coffee alternative, valued for its robust, nutty taste and absence of caffeine. Historically, it has been utilized to facilitate digestion, serve as a diuretic, and promote liver function. This study employed a response surface methodology (RSM) for optimization the ultrasonic-assisted extraction of antioxidant composition from chicory. The optimal extraction conditions (temperature 60 degrees C, extraction duration 4 h, and solid-to-solvent ratio of 1:20 g/mL) resulted in the maximum yield and antioxidant capacity. The metabolic content of chicory extract, prepared under optimal conditions, was found by UPLC-ESI-QTOF/MS, including aesculetin, caftaric acid, caffeoylmalic acid, scopoletin, and chicoric acid. Moreover, chicory extracts derived under identical conditions in two distinct solvents (96% and 50% ethanol) demonstrated vitality and hepatoprotective action in HL-7702 cells. This study may offer a perspective for acquiring potential extracts of chicory as a functional food.

키워드

antioxidantChicory (Cichorium intybus L.)hepatoprotectiveresponse surface methodology (RSM)UPLC-ESI-QTOF/MSOXIDATIVE STRESS
제목
Response Surface Methodology Optimization on Ultrasonic-Assisted Extraction of Chicory (Cichorium intybus L.) and Their UPLC-ESI-QTOF-MS Analysis, Antioxidant and Hepatoprotective Activity
저자
Baiseitova, AizhamalAmzeyeva, UlpanTolepbergen, AiyaUlfanova, YasminaShybyray, YergazyDai, LixiaShang, XiaofeiShah, Abdul BariKim, Jeong YoonJenis, Janar
DOI
10.1002/efd2.70081
발행일
2025-06
유형
Article
저널명
eFood
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