Fluorescence imaging for Fe3+ in Arabidopsis by using simple naphthalene-based ligands

초록

A main source of Fe3+ exposure for mammals is through plant consumption. Thus, sensitive and selective Fe3+ detection in plant tissue is a significant and an urgent need. Although fluorescence probes have been reported for Fe3+ in water, the detection of endogenous biological Fe3+ in plant tissue remains to be refined due to the high background signal and the thickness of the plant tissue that can hamper the effective application of traditional one-photon excitation. To address these issues, we have synthesized naphthalene-based probes, 1 and 1A. Upon an addition of Fe3+ in water-methanol (1 : 1, v/v, pH 7), the fluorescence probes of 1 and 1A were found to dramatically decrease, but no other metal ions had this effect. More interestingly, 1A, which had no diethyl 2,2'-(phenylazanediyl)diacetate moiety, also exhibited high selectivity for Fe3+. These results clearly indicate that the Fe3+ was bound to the nitrogen and oxygen atoms located near the naphthalene moiety. Furthermore, chemical probes of 1 and 1A were embedded into nanofibrous membrane films NF-1 and NF-1A, respectively, prepared by the electrospinning method for use as a portable chemical probe. Fluorescence changes were examined by immersing the films into solutions of various metal ions. The strong fluorescence intensity of both NF-1 and NF-1A dramatically decreased in accordance with concentration of Fe3+ onto the film, which was a "turn-off" system. In contrast, no significant changes of fluorescence intensity were observed compared that of other metal ions, such as Na+, K+, Zn2+, Pb2+, Mn2+, Cu2+, Co2+, Ca2+, Fe2+, and Cd2+. The results indicate that both NF-1 and NF-1A could be used to selectively detect Fe3+. We also investigated the practicality of both 1 and 1A as imaging probes for Fe3+ to operate within living systems like plants. Chemical probes of both 1 and 1A were tested for fluorescence imaging of Fe3+ in Arabidopsis. As expected, the fluorescence probes displayed high fluorescence imaging for Fe3+ in Arabidopsis.

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