Bactericidal effect of a Bacillus complex against Actinobacillus pleuropneumoniae

초록

This study evaluated the bactericidal efficacy of a Bacillus complex composed of Bacillus subtilis YBK-70, Bacillus atrophaeus YBK-W4, and Bacillus circulans YBK-04 (1:1:1, v/v/v) against Actinobacillus pleuropneumoniae (App). A bactericidal efficacy test using the broth dilution method was performed to determine the lowest effective dilution of the Bacillus complex culture medium (BCCM) (4 × 10⁶ colony-forming units/mL) after exposure to the test bacteria for 30 min at 4°C. The BCCM and test bacteria were diluted in suspensions containing low and high levels of organic matter (OM), depending on the treatment conditions. Under low OM conditions, the bactericidal activity of the BCCM against App was observed at all 5.0-fold dilutions. Under high OM conditions, the bactericidal activity of the BCCM against App was observed at all 1.25-fold dilutions. The recommended dilution ratios of the BCCM under low and high OM conditions were 4.0-fold and 1.0-fold, respectively. As the BCCM exhibits bactericidal efficacy against App, it may serve as an effective agent for limiting the spread of bacterial diseases in animals.

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