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- Oh, K.-I.;
- Jin, H.-S.;
- Balasubramaniyam, T.;
- Shin, J.-Y.;
- Choi, S.-R.;
- ... Kim, B.-S.;
- ... Seo, Y.-J.;
- ... Kwon, S.-R.;
- 외 3명
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0SCOPUS
0초록
Developing chemical modifications of DNA to find drug targets is challenging. Here, we incorporated double-methylated guanosine into a DNA duplex and determined its solution structure using NMR and restrained molecular dynamics. The double-methylation of guanosine promotes aberrantly distorted Z-DNA with a widened groove space. This flexible Z-DNA exhibited slow conformational exchange (second time-scale) with a novel helical structure (denoted as tBZ-form). We find three characteristics of the Z-to-tBZ transition: 1) syn-to-syn glycosidic conformation (Z-to-non-B); 2) loss of left-handedness (that is, Z-to-non-Z); 3) entropic gain of the Z-to-tBZ transition. We anticipate that this flexible Z-DNA can be considered a novel target for drug discovery and that the Z-to-tBZ transition will provide new insights into structural diversity. © 2023 Elsevier B.V.
키워드
- 제목
- Entropy-driven conformational transition of flexible Z-DNA to a novel non-B helix by double-methylated guanosine
- 저자
- Oh, K.-I.; Jin, H.-S.; Balasubramaniyam, T.; Shin, J.-Y.; Choi, S.-R.; Seo, Y.J.; Kim, B.-S.; Seo, Y.-J.; Kwon, S.-R.; Kim, N.-K.; Lee, J.-H.
- 발행일
- 2023-08
- 유형
- Article
- 권
- 383