Protein kinase C delta regulates anti-apoptotic alpha B-crystallin in the retina of type 2 diabetes

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32
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33

초록

We investigated the relationship between phosphorylation of alphaB-crystallin (alpha BC) and retinal apoptosis in type 2 diabetes. The retinas of male Otsuka Long-Evans Tokushima fatty (OLETF) rats at 24 and 35 weeks were used as an animal model for type 2 diabetes and sex- and age-matched Long-Evans Tokushima Otsuka (LETO) rats were used as controls. In the retinas of 35-week OLETF rats, the interaction between alpha BC and protein kinase C delta (PKC delta) among the PKC isozymes, alpha BC phosphorylation at Ser45 (S45p-alpha BC), TUNEL-positive apoptotic ganglion cells, several apoptotic signs, and colocalization of S45p-alpha BC and TUNEL significantly increased as compared with other groups while the alpha BC-Bax interaction greatly decreased. These changes were abolished by rottlerin treatment, a highly specific PKC 6 inhibitor. These results suggest that PKC 6 is involved in regulation of anti-apoptotic function of alpha BC in the retina of type 2 diabetes. (C) 2007 Elsevier Inc. All rights reserved.

키워드

alpha BC phosphorylationretinal apoptosisPKC deltarottlerintype 2 diabetesHEAT-SHOCK-PROTEINMIMICKING PHOSPHORYLATIONSIGNALING PATHWAYSGROWTH-FACTORA-CRYSTALLINDNA-DAMAGERAT RETINACELLSACTIVATIONEXPRESSION
제목
Protein kinase C delta regulates anti-apoptotic alpha B-crystallin in the retina of type 2 diabetes
저자
Kim, Y. H.Choi, M. Y.Kim, Y. S.Han, J. M.Lee, J. H.Park, C. H.Kang, S. S.Choi, W. S.Cho, G. J.
DOI
10.1016/j.nbd.2007.07.017
발행일
2007-12
유형
Article
저널명
Neurobiology of Disease
28
3
페이지
293 ~ 303