High concentrations of ascorbic acid induces apoptosis of human gastric cancer cell by p38-MAP kinase-dependent up-regulation of transferrin receptor

Ha, Yu Mi; Park, Min Kyu; Kim, Hye Jung; Seo, Han Geuk; Lee, Jae Heun; Chang, Ki Churl

doi:10.1016/j.canlet.2008.11.020

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High concentrations of ascorbic acid induces apoptosis of human gastric cancer cell by p38-MAP kinase-dependent up-regulation of transferrin receptor

Ha, Yu Mi;
Park, Min Kyu;
Kim, Hye Jung;
Seo, Han Geuk;
Lee, Jae Heun;
외 1명

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초록

We investigated the molecular mechanism by which ascorbic acid (AA) induces apoptosis in human gastric cancer cells, AGS cells. High concentration (more than 5 mM) of AA increased cellular iron uptake by increasing transferrin receptor (TfR) expression and induced AGS cell apoptosis which was inhibited by catalase. Interestingly, p38 mitogen-activated protein kinase (MAPK) inhibitor inhibited the upregulation of TfR and increased cell survival by AA. TfR-siRNA-transfected cells reduced apoptosis by AA. H2O2 increased TfR expression in AGS cells. Taken together, we concluded that high concentration of AA, through H2O2, induces apoptosis of AGS cells by p38-MAPK-dependent upregulation of TfR. (C) 2008 Elsevier Ireland Ltd. All rights reserved.

키워드

Apoptosis; Ascorbic acid; Transferrin receptor; p38-MAPK; Hydrogen peroxide; HYDROGEN-PEROXIDE; OXIDATIVE STRESS; VITAMIN-C; IRON UPTAKE; ENDOTHELIAL-CELLS; RADICAL INTENSITY; DOWN-REGULATION; CULTURE-MEDIUM; IN-VITRO; MAINTENANCE

제목: High concentrations of ascorbic acid induces apoptosis of human gastric cancer cell by p38-MAP kinase-dependent up-regulation of transferrin receptor

저자: Ha, Yu Mi; Park, Min Kyu; Kim, Hye Jung; Seo, Han Geuk; Lee, Jae Heun; Chang, Ki Churl

DOI: 10.1016/j.canlet.2008.11.020

발행일: 2009-05-08

유형: Article

저널명: Cancer Letters

권: 277

호: 1

페이지: 48 ~ 54