쉬땅나무에서 분리한 Lactiplantibacillus plantarum SSDan-7의 프로바이오틱스로서의 물리화학적 특성

초록

Twenty-five Lactobacillus SSDs and other lactic acid bacteria isolated from Sorbaria sorbifolia were distributed through the National Institute of Biological Resources (NIBR) to evaluate functions as probiotics. Among them, Lactobacillus spp. SSD-8, SSD-9, and SSDan-7 were initially selected due to their higher emulsification ability against kerosene. Lactobacillus spp. SSD-8 and SSDan-7 strains exhibited higher values than SSD-9 across extraction temperatures and were selected for further study. Lactobacillus spp. SSDan-7 was finally selected based on cultural conditions, extraction conditions, and thermal stability, and SSDan-7 was identified as Lactiplantibacillus plantarum by 16S rDNA sequencing. When sucrose was used as a carbon source, L. plantarum SSDan-7 showed the highest biosurfactant production ability. Critical micelle concentration (CMC) of SSDan-7 was showed by similar values with L. plantarum KACC 12404 and K9. Autoaggregation of SSDan -7 was observed at the highest value at 20 h, which was exhibited by lower activity compared with L. plantarum KACC 12404 and K9. Coaggregation of SSDan-7 was continuously increased until 20 h and 24 h against S. aureus and S. Typhimurium, respectively, which showed similar values with L. plantarum KACC 12404 and K9. SSDan-7 as well as almost SSDs have excellent adhesion to mucin originated from intestinal epithelial cells. Cell surface hydrophobicity was highest in chloroform, which indicates electronic donation. Therefore, given its high industrial applicability, L. plantarum SSDan-7 shows strong potential as a probiotic.

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