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초록
Distinction of Bacillus cereus from other closely related bacilli is challenging and new efficient methods are continually demanded. From our previous work on RAPD profiles of bacilli, we found a possibility that B. cereus strains could be distinguished from other bacilli. In this work, RAPD-PCR profiles of B. cereus strains were obtained using a 10-mer (S30) as a primer, and a B. cereus specific 0.91-kb band was produced from all tested strains. The RAPD-PCR procedure also successfully detected B. cereus from spiked cheonggukjang when B. cereus cells were present at more than 10(2)/g sample.
키워드
Bacillus cereus; food poisoning; rapid detection; RAPD-PCR; IDENTIFICATION
- 제목
- A RAPD-PCR Method for the Rapid Detection of Bacillus cereus
- 저자
- Lee, Jiyeon; Kwon, Gun-Hee; Park, Jae-Yong; Park, Cheon-Seok; Kwon, Dae Young; Lim, Jinkyu; Kim, Jong Sang; Kim, Jeong Hwan
- 발행일
- 2011-03
- 유형
- Article
- 권
- 21
- 호
- 3
- 페이지
- 274 ~ 276
- 언어
- ENG
- 출판사
- KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY
- 발행국가
- 대한민국
- 분량
- 3 페이지
- ISSN
- E 1738-8872
P 1017-7825