Ultraviolet photodissociation circular dichroism spectroscopy of protonated L-phenylalanyl-L-alanine in a cryogenic ion trap
  • Yoo, Il Tae
  • Eun, Han Jun
  • Min, Ahreum
  • Jeon, Chang Wook
  • Jeong, Jinho
  • 외 2명
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초록

We obtained ultraviolet photodissociation (UVPD) circular dichroism (CD) spectra of protonated L-phenylalanyl-L-alanine (L-H(+)PheAla) near the origin band of the S-0-S-1 transition using cryogenic ion spectroscopy. Infrared (IR) ion-dip, IR-UV hole burning (HB) and UV-UV HB spectra showed that L-H(+)PheAla existed as two different conformers in a cryogenic ion trap, and they had nearly identical peptide backbones but different conformations in the Phe side chain. The UVPD CD spectra revealed that the two conformers had opposite CD signs and significantly different CD magnitudes from each other. These results demonstrate that the CD value of l-H(+)PheAla near the origin band is strongly influenced by the conformation of the Phe side chain.

키워드

HOLE-BURNING SPECTROSCOPYELECTRONIC SPECTROSCOPYGAS-PHASECOLDUVPROTEINSSPECTRADIMER
제목
Ultraviolet photodissociation circular dichroism spectroscopy of protonated L-phenylalanyl-L-alanine in a cryogenic ion trap
저자
Yoo, Il TaeEun, Han JunMin, AhreumJeon, Chang WookJeong, JinhoHeo, JiyoungKim, Nam Joon
DOI
10.1039/d1cp04030h
발행일
2021-11
유형
Article
저널명
Physical Chemistry Chemical Physics
23
42
페이지
24180 ~ 24186