Proteomic analysis of compatible and incompatible interactions of wheat with Puccinia triticina

초록

Resistant (WL711+Lr57) and susceptible (WL711-Lr57) near isogenic lines of wheat were used to unravel the molecular basis of seedling resistance conferred by Lr57 gene during wheat-Puccinia triticina (race 77-5) interaction. Differentially expressed proteins during incompatible and compatible interactions were identified through 2D-PAGE and their peptide mass fingerprint was determined by MALDI TOF. In silico functional annotation revealed the presence of diverse categories of proteins associated with defense, photosynthesis, nucleotide repair, DNA replication, protein synthesis and transposable elements. For the first time, the differential expression of a nucleotide repair related protein, MMS19, during wheat-Puccinia interaction is being reported in this study. The proteomic analysis of 96 hpi WL711-Lr57 revealed the negative regulation of JA, SA and ET mediated pathways, thus accounting for its susceptibility. Transcriptomic validation was performed for a set of selected potential candidate proteins through qRT-PCR. The identified proteins possess the potential to be exploited in development of novel wheat varieties tolerant to leaf rust at seedling stage. (C) 2016 Elsevier Ltd. All rights reserved.

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