상세 보기
- Lee, E.-G.;
- Kim, J.-H.;
- Shin, Y.-S.;
- Shin, G.-W.;
- Kim, Y.-R.;
- ... Kim, Y.-H.;
- ... Kim, G.-S.;
- ... Suh, M.-D.;
- ... Jung, T.-S.;
- 외 3명
WEB OF SCIENCE
0SCOPUS
30초록
Protein profiles of two isolates of Neospora caninum (KBA-2 and JPA1) and Toxoplasma gondii RH strain were investigated by proteomic approach. Approximately, 78% of protein spots on two-dimensional gel electrophoresis (2-DE) profiles and 80% of antigen spots on 2-DE immunoblotting profiles were exhibited to share the same pI and Mr between KBA-2 and JPA1 of N. caninum. On the other hand, a total of 30 antigen spots of T. gondii were recognized on 2-DE immunoblotting profile using rabbit antiserum against N. caninum KBA-2. A number of homologue proteins, such as heat shock protein 70, tubulin α- and β-chain, putative protein disulfide isomerase, actin, enolase and 14-3-3 protein homologue are believed as the conserved proteins in both N. caninum and T. gondii. On the contrary, NcSUB1, NcGRA2 and NCDG1 (NcGRA7) might be the species-specific proteins for N. caninum tachyzoites. The present study showed that the high degree of similarity between N. caninum isolates (KBA-2 and JPA1), whereas large differences between N. caninum and T. gondii were noticed by proteome comparisons. © 2004 Elsevier B.V. All rights reserved.
키워드
- 제목
- Application of proteomics for comparison of proteome of Neospora caninum and Toxoplasma gondii tachyzoites
- 저자
- Lee, E.-G.; Kim, J.-H.; Shin, Y.-S.; Shin, G.-W.; Kim, Y.-R.; Palaksha, K.J.; Kim, D.-Y.; Yamane, I.; Kim, Y.-H.; Kim, G.-S.; Suh, M.-D.; Jung, T.-S.
- 발행일
- 2005-02
- 유형
- Article
- 권
- 815
- 호
- 1-2
- 페이지
- 305 ~ 314