급성 및 만성 에탄올 유도 간 손상 마우스 모델에서 단감(Diospyros kaki Thunb. cv. Fuyu) 추출물의 보호 효과

초록

This study evaluated the antioxidant activity and alcohol metabolism-modulating effects of Diospyros kaki Thunb. cv. Fuyu aqueous extract using in vitro assays and ethanol-induced mouse models. Four samples—aqueous extract of persimmon flesh (PF), aqueous extract of enzyme-treated persimmon flesh (EPF), aqueous extract of persimmon peel (PP), and aqueous extract of enzyme-treated persimmon peel (EPP)—were analyzed for total phenolic and tannin contents, 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activities, and alcohol-metabolizing enzyme activities. Among these, the flesh-based extracts (PF and EPF) exhibited higher acetaldehyde dehydrogenase (ALDH) activity than the peel-based extracts and were therefore selected as primary candidate materials. Subsequent HepG2 cell experiments showed that EPF improved cell viability and reduced ethanol-induced reactive oxygen species (ROS) production more effectively than PF. In an acute ethanol-induced mouse model, EPF also showed a stronger effect on lowering serum alcohol and acetaldehyde concentrations. Based on these results, EPF was selected as the final candidate material for the chronic ethanol-induced mouse study. In a chronic ethanol-induced model, EPF ameliorated serum markers of liver injury, including aspartate aminotransferase (AST), alanine aminotransferase (ALT), and alkaline phosphatase (ALP), and attenuated mitochondrial oxidative stress by restoring membrane potential and increasing ATP production. Finally, HPLC analysis confirmed protocatechuic acid as one of the detectable phenolic compounds in the EPF fraction.

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