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초록
The Z alpha domain of human ADAR1 (Z alpha(ADAR1)) preferentially binds Z-DNA rather than B-DNA with high binding affinity. Z alpha(ADAR1) binds to the Z-conformation of both non-CG-repeat DNA duplexes and a d(CGCGCG)(2) duplex similarly. We performed NMR experiments on complexes between the Z alpha(ADAR1) and non-CG-repeat DNA duplexes, d(CACGTG)(2) or d(CGTACG)(2), with a variety of protein-DNA molar ratios. Comparison of these results with those from the analysis of d(CGCGCG)(2) in the previous study suggests that Za(ADAR1) exhibits the sequence preference of d(CGCGCG)(2) >> d(CACGTG)(2) > d(CGTACG)(2) through multiple sequence discrimination steps during the B-Z transition. (C)2010 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
키워드
- 제목
- Sequence discrimination of the Z alpha domain of human ADAR1 during B-Z transition of DNA duplexes
- 저자
- Seo, Yeo-Jin; Ahn, Hee-Chul; Lee, Eun-Hae; Bang, Jongchul; Kang, Young-Min; Kim, Hee-Eun; Lee, Yeon-Mi; Kim, Kyungmin; Choi, Byong-Seok; Lee, Joon-Hwa
- 발행일
- 2010-10-22
- 유형
- Article
- 저널명
- FEBS Letters
- 권
- 584
- 호
- 20
- 페이지
- 4344 ~ 4350