Establishment of withdrawal time and analysis of gentamicin residue in edible tissues of broiler chickens following intramuscular injection

초록

This study investigated gentamicin (GEN) residues in edible tissues of broiler chickens following intramuscular injection and established the withdrawal time (WT) of GEN in poultry edible tissues. Thirty healthy broiler chickens were intramuscularaly injected with GEN at doses of 2.0 mg/kg body weight (BW) once. After drug administration, tissue samples were collected from six broiler chickens at 7, 14, 21, 28, and 42 days post-treatment. GEN residue concentrations in poultry edible tissues were determined using LC-MS/MS. The correlation coefficient values ranged from 0.9976 to 0.9998, and the limits of detection (LOD) and quantification (LOQ) were 0.003-0.008 mg/kg and 0.01-0.025 mg/kg, respectively. Recoveries in muscle, liver, kidney, and fat tissues were 50.39-74.57%, 50.35-66.24%, 65.57-74.23% and 81.62-111.31%, respectively, with coefficients of variation less than 14.17%. In broiler chickens intramuscularally injected with GEN, GEN concentrations in muscle, injection site, liver, and fat samples were below the LOQ on day 14, 21, 42, and 14 post-treatment, respectively. However, GEN was detected in kidney samples at levels above the LOQ up to day 42 after drug treatment. According to the European Medicines Agency’s guideline on the determination of withdrawal periods for edible tissues, the withdrawal period for GEN in liver and kidney samples were established as 38 and 39 days, respectively. In conclusion, the developed analytical method is suitable for detecting GEN in poultry tissues, and the estimated WT of GEN will contribute to ensuring the safety of poultry products.

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