ScholarWorks@경상국립대학교

초록

Enhancing intestinal calcium absorption in the elderly is essential for reducing the risk of osteoporosis. The purpose of this study was to manufacture calcium-containing casein phosphopeptide (CPP)-chitosan oligosaccharide (CSO) nanocomplexes and determine their physicochemical properties (e.g., particle size) and functional characteristics (e.g., intestinal calcium absorption and entrapment efficiency). CPP-CSO nanocomplexes with particle sizes of less than 200 nm were successfully formed. The particle size of the CPP-CSO nanocomplexes increased with increasing CPP concentration, whereas their zeta potential value decreased. Increasing the pH from 5.5 to 6.6 led to a decrease in the particle size, and the zeta potential values decreased accordingly. Caco-2 cells exhibited no cytotoxicity at the various nanocomplex concentrations. The intestinal calcium absorption of the CPP-CSO nanocomplexes increased with a decrease in CPP concentration and an increase in pH. A negative correlation was found between calcium absorption and the particle size of CPP-CSO nanocomplexes. In conclusion, the CPP concentration and pH are manufacturing factors that affect the physicochemical properties of CPP-CSO nanocomplexes.

키워드