Differential interleukin-1 beta induction by uropathogenic Escherichia coli correlates with its phylotype and serum C-reactive protein levels in Korean infants

Abstract

Urinary tract infection (UTI) is one of the most common bacterial infections in infants less than age 1 year. UTIs frequently recur and result in long-term effects include sepsis and renal scarring. Uropathogenic Escherichia coli (UPEC), the most prevalent organism found in UTIs, can cause host inflammation via various virulence factors including hemolysin and cytotoxic necrotizing factors by inducing inflammatory cytokines such as interleukin (IL)-1 beta. However, the ability of each UPEC organism to induce IL-1 beta production may differ by strain. Furthermore, the correlation between differential IL-1 beta induction and its relevance in pathology has not been well studied. In this study, we isolated UPEC from children under age 24 months and infected bone-marrow derived macrophages with the isolates to investigate secretion of IL-1 beta. We found that children with higher concentrations of C-reactive protein (CRP) were more likely to harbor phylotype B2 UPEC strains that induced more IL-1 beta production than phylotype D. We also observed a significant correlation between serum CRP level and in vitro IL-1 beta induction by phylotype B2 UPEC bacteria. Our results highlight the diversity of UPEC in terms of IL-1 beta induction capacity in macrophages and suggest a potential pathogenic role in UTIs by inducing inflammation in infants.