Lactiplantibacillus plantarum LM1001 배양물이 반추위 내 발효특성과 온실가스 발생량에 미치는 영향

Abstract

This study aimed to estimate the effects of Lactiplantibacillus plantarum LM1001 culture (LPC) on fermentation characteristics and greenhouse gas emissions in the rumen. Pure starch and cellulose were mixed at a 1:1 ratio, and used as the substrate for 12 h and 24 h of rumen incubations. Rumen fluid was collected from two cannulated Hanwoo cows just before morning feeding and mixed with Van Soest medium at a 1:2 ratio for the rumen buffer. The substrate (0.3 g) and rumen buffer (30 mL) were placed into the incubation bottles (n = 4) with the additions of 0%, 10%, and 20% of LPC based on the rumen buffer (v/v). After the incubation times, total gas was measured and sub-sampled for CO2 and CH4 analyses. Then, the bottle content was centrifuged for in vitro digestibilities of dry matter (IVDMD) and organic matter (IVOMD), and rumen fermentation characteristics. By increases of LPC supplementation levels, acetate content decreased (Linear, p = 0.027) at 12 h of incubation time and propionate content increased (Linear, p = 0.015), resulting in a decrease of the acetate to propionate ratio (Linear, p = 0.035). And, total gas emission (mL/g DM) in 20% LPC was higher (p < 0.05) than that in the control at 12 h incubation time, whereas CH4 and CO2 (mL/g DM, DMD and OMD) emissions were lower (p < 0.05) than those in the control. In contrast, both greenhouse gas emissions (mL/g DM, DMD, and OMD) in LPC supplemented treatments were higher (p < 0.05) than those in the control at 24 h incubation time. Therefore, this study concluded that LPC supplementation has benefits on greenhouse gas mitigation in the rumen at 12 h incubation time without adverse effects on nutrient digestibility.