Isoeugenol의 넙치(Paralichthys olivaceus) 에 대한 진정,마취 및 독성효과 검토

Abstract

Olive flounder (Paralichthys olivaceus) is the major mariculture fish in Korea. The annual aquaculture production of olive flounder in Korea during the period of 2003 was 300,000 ton (2002 Statistics, Ministry of Maritime Affairs & Fisheries, Korea Government). Anesthetics is very necessary in aquaculture to minimize stress and damage during harvesting, grading, transportation, spawning induction and handling to fish. In the present study, isoeugenol as new anesthetic in marine fish, especially olive flounder, was examined to know the efficacy and proper concentration. As a result, olive flounder adult was exhibited sedation at 5 ppm at 10 and 15oC, and 7.5 ppm at 20oC, respectively. Anesthesia was required at least 10, 7.5 and 10 ppm at 10oC, 15oC and at 20oC, respectively. In case of fry, the effect of sedation was observed from 2.5 ppm at 10oC and 5 ppm at 15 and 20oC, respectively. Anesthesia was observed from 2.5 ppm at 10oC, 5 ppm at 15 and 20oC, respectively. In acute toxicity test, it was impossible to explore LD50 with the concentration of isoeugenol adult at 15oC used, but over immersion volume of 15 ppm at 15oC was observed mortality in fry. Based on the present study, isoeugenol was identified as a safe and active anesthetic to olive flounder.