Influence of satellite cell purity on amino acid composition and myotube characteristics of cultured chicken tissue

Abstract

This study aimed to evaluate the effect of satellite cell (SC) purity on myotube formation and amino acid compositionin cultured chicken tissues, compared with chicken muscle tissue. The results showed that a higher SC purity promoteduniform myotube formation and reduced fibroblast interference, resulting in a more consistent amino acid profile. Conversely, a lower SC purity increased fibroblast proliferation, altered the metabolic environment, and led to elevatedlevels of histidine and certain nonessential amino acids. Additionally, compared to chicken meat, the cultured tissue hadsignificantly lower levels of glycine, serine, alanine, and proline, whereas higher levels of glutamic acid, aspartic acid,and branched-chain amino acids (p<0.05). Amino acid profiling revealed significant variations in glycine, alanine, histidine,and arginine with SC purity, which are linked to taste (sweetness and bitterness) and essential nutritional functions. Overall, these results emphasize the need to optimize the SC isolation and culture conditions to enhance the structuraland nutritional equivalence of cultured chicken meat, ensuring that it is consistent with conventional chicken meat inall aspects.