Elevated urinary 8-isoprostaglandin F2α in females with Graves' hyperthyroidism

Abstract

In this study, we investigate how oxidative stress alters the urinary F2-isoprostanes' level and we examine the correlation between 8-isoPGF2α and thyroid hormones in female patients with Graves' hyperthyroidism. We quantitatively determined the concentrations of urinary F2-isoprostanes using gas chromatography-mass spectrometry in the selected ion-monitoring mode. We recruited individuals in the following three groups of subjects for this study: (1) female hyperthyroidism patients (n=14, 21-71 years), (2) female hypothyroidism patients (n=16, 16-66 years), and (3) female age-matched normal controls (n=10, 20-61 years). The average concentration of 8-iso prostaglandin F2α (8-isoPGF 2α) in hyperthyroidism patients was significantly higher than that in the healthy controls (P<0.05). The concentration of 8-isoPGF 2α in hypothyroidism patients was similar to that in normal controls. Although the level of 8-isoPGF2α in two of the patients was slightly elevated, the P value was not significant (NS). Our data suggest that the increased level of urinary 8-isoPGF2α may reflect possible oxidation injuries in hyperthyroidism patients due to the high level of thyroid hormones. Therefore, the elevated 8-isoPGF2α in patients could be used as an important biomarker for hyperthyroidism disease. © 2004 Published by Elsevier Ltd.