Quantitative Analysis of Caffeoylquinic Acid Derivatives in Methanolic Extract of Ainsliaea Acerifolia Leaves Using HPLC-DADQuantitative Analysis of Caffeoylquinic Acid Derivatives in Methanolic Extract of Ainsliaea Acerifolia Leaves Using HPLC-DAD
- Other Titles
- Quantitative Analysis of Caffeoylquinic Acid Derivatives in Methanolic Extract of Ainsliaea Acerifolia Leaves Using HPLC-DAD
- Authors
- 강현철; 강주영; 노혜령; 김봉규
- Issue Date
- Oct-2024
- Publisher
- 경상국립대학교 농업생명과학연구원
- Keywords
- HPLC-DAD; Chlorogenic acid; Isochlorogenic acid A; and 1; 5-dicaffeoylquinic acid; Ainsliaea acerifolia leaves
- Citation
- 농업생명과학연구, v.58, no.5, pp 71 - 79
- Pages
- 9
- Indexed
- KCI
- Journal Title
- 농업생명과학연구
- Volume
- 58
- Number
- 5
- Start Page
- 71
- End Page
- 79
- URI
- https://scholarworks.gnu.ac.kr/handle/sw.gnu/74576
- ISSN
- 1598-5504
2383-8272
- Abstract
- Ainsliaea acerifolia leaves are registered with the Ministry of Food and Drug Safety as edible herbal materials in Korea, and research is underway to explore their potential in developing functional foods, cosmetics, and pharmaceuticals. In this study, we developed an analytical method using HPLC-DAD to quantify three key compounds—chlorogenic acid, isochlorogenic acid A, and 1,5-dicaffeoylquinic acid—in A. acerifolia leaves extract. This method has been optimized and validated for specificity, accuracy, precision, limit of quantification (LOQ), and linearity. The correlation coefficients (r²) for the calibration curves exceeded 0.9962. The limits of detection (LOD) and quantification (LOQ) were 0.3012 and 0.9128 µg/mL for chlorogenic acid, 0.1182 and 0.3582 µg/mL for isochlorogenic acid A, and 0.2342 and 0.7098 µg/mL for 1,5-dicaffeoylquinic acid, respectively. The net recovery rates for accuracy testing were 105.13% for chlorogenic acid, 105.37% for isochlorogenic acid A, and 100.37% for 1,5-dicaffeoylquinic acid. All parameters assessed with this newly developed method fell within the acceptable ranges specified by ICH guidelines. These findings demonstrate that the method is robust and reliable for accurately identifying and quantifying chlorogenic acid, isochlorogenic acid A, and 1,5-dicaffeoylquinic acid in both routine analysis and large-scale extraction process of A. acerifolia leaves.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - 자연과학계열 > 산림자원학과 > Journal Articles
- 자연과학계열 > 산림융복합학과 > Journal Articles
- 농업생명과학대학 > 환경산림과학부 > Journal Articles

Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.