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Quantitative Analysis of Caffeoylquinic Acid Derivatives in Methanolic Extract of Ainsliaea Acerifolia Leaves Using HPLC-DADQuantitative Analysis of Caffeoylquinic Acid Derivatives in Methanolic Extract of Ainsliaea Acerifolia Leaves Using HPLC-DAD

Other Titles
Quantitative Analysis of Caffeoylquinic Acid Derivatives in Methanolic Extract of Ainsliaea Acerifolia Leaves Using HPLC-DAD
Authors
강현철강주영노혜령김봉규
Issue Date
Oct-2024
Publisher
경상국립대학교 농업생명과학연구원
Keywords
HPLC-DAD; Chlorogenic acid; Isochlorogenic acid A; and 1; 5-dicaffeoylquinic acid; Ainsliaea acerifolia leaves
Citation
농업생명과학연구, v.58, no.5, pp 71 - 79
Pages
9
Indexed
KCI
Journal Title
농업생명과학연구
Volume
58
Number
5
Start Page
71
End Page
79
URI
https://scholarworks.gnu.ac.kr/handle/sw.gnu/74576
ISSN
1598-5504
2383-8272
Abstract
Ainsliaea acerifolia leaves are registered with the Ministry of Food and Drug Safety as edible herbal materials in Korea, and research is underway to explore their potential in developing functional foods, cosmetics, and pharmaceuticals. In this study, we developed an analytical method using HPLC-DAD to quantify three key compounds—chlorogenic acid, isochlorogenic acid A, and 1,5-dicaffeoylquinic acid—in A. acerifolia leaves extract. This method has been optimized and validated for specificity, accuracy, precision, limit of quantification (LOQ), and linearity. The correlation coefficients (r²) for the calibration curves exceeded 0.9962. The limits of detection (LOD) and quantification (LOQ) were 0.3012 and 0.9128 µg/mL for chlorogenic acid, 0.1182 and 0.3582 µg/mL for isochlorogenic acid A, and 0.2342 and 0.7098 µg/mL for 1,5-dicaffeoylquinic acid, respectively. The net recovery rates for accuracy testing were 105.13% for chlorogenic acid, 105.37% for isochlorogenic acid A, and 100.37% for 1,5-dicaffeoylquinic acid. All parameters assessed with this newly developed method fell within the acceptable ranges specified by ICH guidelines. These findings demonstrate that the method is robust and reliable for accurately identifying and quantifying chlorogenic acid, isochlorogenic acid A, and 1,5-dicaffeoylquinic acid in both routine analysis and large-scale extraction process of A. acerifolia leaves.
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자연과학계열 > 산림자원학과 > Journal Articles
자연과학계열 > 산림융복합학과 > Journal Articles
농업생명과학대학 > 환경산림과학부 > Journal Articles

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Kim, Bong Gyu
농업생명과학대학 (환경산림과학부)
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