Antibacterial Activity of Korean Resource Plant Extracts against Helicobacter pylori and Inhibitory Effect of IL-8 Secretion in Gastric Epithelial Cells

Abstract

Helicobacter pylori are known as a causative agent of gastritis, gastric duodenum and peptic ulcer, and gastric cancer, and multipledrug use is associated with various side effects in patients. The discovery of antibacterial substances against H. pylori from Koreanresource plants is an important substitute for antibiotics. 52 species of Korean resource plants were collected and extracted with 50%ethanol, and antibacterial activity against H. pylori was measured using the disk diffusion method. The toxicity of plant extracts tohuman gastric adenocarcinoma(AGS) cells was measured by MTT assay, and the level of IL-8 secreted when gastric epithelial cellswere inoculated with H. pylori was measured. As a result of measuring the antibacterial activity of H. pylori, antibacterial activity wasconfirmed in 38 plant extracts. The plant species with the strongest antibacterial activity were Chrysanthemum indicum, Rheumrhabarbarum, Patrinia scabiosaefolia and Petasites japonicus. C. indicum was not cytotoxic to H. pyroli-infected AGS cells and showedanti-inflammatory effects. This study's results can be used to develop healthy, functional foods and medical materials.