CRISPR/Cas12a-based biosensors for environmental monitoring and diagnostics

Abstract

Contaminants, such as nucleic acids or toxic small molecules, threaten both human health and the environment. Precise and highly sensitive identification of such contaminants holds paramount importance across diverse domains, including safeguarding food safety, facilitating diagnostics, and monitoring environmental conditions. Traditional methodologies, encompassing spectroscopy, chromatography, sequencing, and metagenomics, have served pivotal roles in detection processes. Nevertheless, these methods have encountered recurring challenges related to sensitivity, specificity, and portability. This review focuses on the groundbreaking CRISPR/Cas-based biosensors. These biosensors leverage the incredible precision and programmability of the CRISPR/Cas system to recognize specific targets. Here, we comprehensively assess the fundamental mechanisms that enable specific and accurate detection, covering topics from guide RNA design to optimization of collateral cleavage activity. The versatility of CRISPR/Cas12a biosensors becomes evident through their diverse applications. These applications encompass medical diagnostics, food safety, and environmental monitoring. The transition from conventional detection methods to biosensors and ultimately to CRISPR/Cas-biosensors represents a significant milestone in diverse contaminant detection. By incorporating molecular biology, nanotechnology, and bioinformatics, these biosensors have the potential to reshape the landscape of water safety, diagnostics, and environmental monitoring. CRIPSR-Cas diagnostics is a transformative technology that paves the way for a safer and healthier future for the environment and human life. © 2024 The Authors