Role of Wnt Signaling During In-Vitro Bovine Blastocyst Development and Maturation in Synergism with PPAR delta Signaling
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Sidrat, Tabinda | - |
dc.contributor.author | Khan, Abdul Aziz | - |
dc.contributor.author | Idrees, Muhammad | - |
dc.contributor.author | Joo, Myeong-Don | - |
dc.contributor.author | Xu, Lianguang | - |
dc.contributor.author | Lee, Kyeong-Lim | - |
dc.contributor.author | Kong, Il-Keun | - |
dc.date.accessioned | 2022-12-26T13:00:48Z | - |
dc.date.available | 2022-12-26T13:00:48Z | - |
dc.date.created | 2022-12-12 | - |
dc.date.issued | 2020-04 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gnu/handle/sw.gnu/6771 | - |
dc.description.abstract | Wnt/beta-catenin signaling plays vital role in the regulation of cellular proliferation, migration, stem cells cell renewal and genetic stability. This pathway is crucial during the early developmental process; however, the distinct role of Wnt/beta-catenin signaling during pre-implantation period of bovine embryonic development is obscure. Here, we evaluated the critical role of Wnt/beta-catenin pathway in the regulation of bovine blastocyst (BL) development and hatching. 6 bromoindurbin-3'oxime (6-Bio) was used to stimulate the Wnt signaling. Treatment with 6-Bio induced the expression of peroxisome proliferator-activated receptor-delta (PPAR delta). Interestingly, the PPAR delta co-localized with beta-catenin and form a complex with TCF/LEF transcription factor. This complex potentiated the expression of several Wnt directed genes, which regulate early embryonic development. Inhibition of PPAR delta with selective inhibitor 4-chloro-N-(2-{[5-trifluoromethyl]-2-pyridyl]sulfonyl}ethyl)benzamide (Gsk3787) severely perturbed the BL formation and hatching. The addition of Wnt agonist successfully rescued the BL formation and hatching ability. Importantly, the activation of PPAR delta expression by Wnt stimulation enhanced cell proliferation and fatty acid oxidation (FAO) metabolism to improve BL development and hatching. In conclusion, our study provides the evidence that Wnt induced PPAR delta expression co-localizes with beta-catenin and is a likely candidate of canonical Wnt pathway for the regulation of bovine embryonic development. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | MDPI | - |
dc.subject | ACTIVATED-RECEPTOR-DELTA | - |
dc.subject | BETA-CATENIN | - |
dc.subject | EXPRESSION | - |
dc.subject | MOUSE | - |
dc.subject | GENE | - |
dc.subject | CYCLOOXYGENASE-2 | - |
dc.subject | IMPLANTATION | - |
dc.subject | PROSTACYCLIN | - |
dc.subject | PATHWAY | - |
dc.subject | GAMMA | - |
dc.title | Role of Wnt Signaling During In-Vitro Bovine Blastocyst Development and Maturation in Synergism with PPAR delta Signaling | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kong, Il-Keun | - |
dc.identifier.doi | 10.3390/cells9040923 | - |
dc.identifier.wosid | 000535559500134 | - |
dc.identifier.bibliographicCitation | CELLS, v.9, no.4 | - |
dc.relation.isPartOf | CELLS | - |
dc.citation.title | CELLS | - |
dc.citation.volume | 9 | - |
dc.citation.number | 4 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.subject.keywordPlus | ACTIVATED-RECEPTOR-DELTA | - |
dc.subject.keywordPlus | BETA-CATENIN | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | MOUSE | - |
dc.subject.keywordPlus | GENE | - |
dc.subject.keywordPlus | CYCLOOXYGENASE-2 | - |
dc.subject.keywordPlus | IMPLANTATION | - |
dc.subject.keywordPlus | PROSTACYCLIN | - |
dc.subject.keywordPlus | PATHWAY | - |
dc.subject.keywordPlus | GAMMA | - |
dc.subject.keywordAuthor | pre-implantation | - |
dc.subject.keywordAuthor | Wnt | - |
dc.subject.keywordAuthor | beta-catenin | - |
dc.subject.keywordAuthor | bovine blastocyst | - |
dc.subject.keywordAuthor | PPAR delta | - |
dc.subject.keywordAuthor | FAO metabolism | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
Gyeongsang National University Central Library, 501, Jinju-daero, Jinju-si, Gyeongsangnam-do, 52828, Republic of Korea+82-55-772-0533
COPYRIGHT 2022 GYEONGSANG NATIONAL UNIVERSITY LIBRARY. ALL RIGHTS RESERVED.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.