PRDX4 overexpression is associated with poor prognosis in gastric cancer
DC Field | Value | Language |
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dc.contributor.author | Park, Sun Yi | - |
dc.contributor.author | Lee, Young-Joon | - |
dc.contributor.author | Park, Jiho | - |
dc.contributor.author | Kim, Tae-Han | - |
dc.contributor.author | Hong, Soon-Chan | - |
dc.contributor.author | Jung, Eun-Jung | - |
dc.contributor.author | Ju, Young-Tae | - |
dc.contributor.author | Jeong, Chi-Young | - |
dc.contributor.author | Park, Hee Jin | - |
dc.contributor.author | Ko, Gyung Hyuck | - |
dc.contributor.author | Song, Dae Hyun | - |
dc.contributor.author | Park, Miyeong | - |
dc.contributor.author | Yoo, Jiyun | - |
dc.contributor.author | Jeong, Sang-Ho | - |
dc.date.accessioned | 2022-12-26T12:47:41Z | - |
dc.date.available | 2022-12-26T12:47:41Z | - |
dc.date.issued | 2020-05 | - |
dc.identifier.issn | 1792-1074 | - |
dc.identifier.issn | 1792-1082 | - |
dc.identifier.uri | https://scholarworks.gnu.ac.kr/handle/sw.gnu/6669 | - |
dc.description.abstract | Peroxiredoxin IV (PRDX4) is a multifunctional protein that is involved in cell protection against oxidative injury, regulation of cell proliferation, modulation of intracellular signaling, and the pathogenesis of tumors. We previously conducted a proteomic analysis to investigate tumor-specific protein expression in gastric cancer. The aim of the present study was to investigate whether PRDX4 could be a marker of poor prognosis in patients with gastric cancer. Immunohistochemistry was used to validate PRDX4 as a prognostic marker for gastric cancer. Short hairpin RNA (shRNA)-mediated knockdown of PRDX4 expression in AGS cells and MKN28 cells was used for functional studies, and PRDX4 overexpression in PRDX4-depleted cells was used for knock-in studies. Based on immunohistochemistry data, TNM stage and PRDX4 were independent prognostic factors in the Cox proportional hazard model (P<0.05). In the survival analysis, the PRDX4-overexpressing group demonstrated significantly worse survival than the PRDX4-underexpression group (P<0.01). In vitro, knockdown of PRDX4 expression by shRNA caused a significant decrease in cancer invasion. Conversely, overexpression of PRDX4 in PRDX4-depleted cancer cells promoted migration and invasion. By measuring the expression of EMT-related genes, we found that E-cadherin was increased in shPRDX4 cells compared with control shMKN28 cells, and snail and slug were decreased in shPRDX4-1 cells compared with sh-control cells. Furthermore, the expression levels of these genes could be recovered in rescue experiments. In conclusion, the results of the present study suggested that PRDX4 is a marker of poor prognosis in gastric cancer and that PRDX4 is associated with cancer cell migration and invasion via EMT. | - |
dc.format.extent | 9 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | SPANDIDOS PUBL LTD | - |
dc.title | PRDX4 overexpression is associated with poor prognosis in gastric cancer | - |
dc.type | Article | - |
dc.publisher.location | 그리이스 | - |
dc.identifier.doi | 10.3892/ol.2020.11468 | - |
dc.identifier.scopusid | 2-s2.0-85082778572 | - |
dc.identifier.wosid | 000528714800018 | - |
dc.identifier.bibliographicCitation | ONCOLOGY LETTERS, v.19, no.5, pp 3522 - 3530 | - |
dc.citation.title | ONCOLOGY LETTERS | - |
dc.citation.volume | 19 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 3522 | - |
dc.citation.endPage | 3530 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Oncology | - |
dc.relation.journalWebOfScienceCategory | Oncology | - |
dc.subject.keywordPlus | PEROXIREDOXINS | - |
dc.subject.keywordPlus | MECHANISMS | - |
dc.subject.keywordPlus | CHEMOTHERAPY | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordAuthor | stomach neoplasm | - |
dc.subject.keywordAuthor | peroxiredoxin IV | - |
dc.subject.keywordAuthor | biomarkers | - |
dc.subject.keywordAuthor | prognosis | - |
dc.subject.keywordAuthor | survival analysis | - |
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