Pelargonidin suppresses adipogenesis in 3T3-L1 cells through inhibition of PPAR-gamma signaling pathway

Abstract

Pelargonidin is a natural compound that exists widely in fruits, and exerts antioxidant, anti-atherosclerotic, anti-inflammatory, anti-hyperglycemic, and anti-diabetic activities. However, there have not been any studies concerning its anti-obesity potential to date. Therefore, we evaluated the anti-obesity potential of pelargonidin via inhibition of adipogenesis in 3T3-L1 cells. The cellular oil droplet content was decreased to 68.14%, 56.75%, and 48.39% and triglyceride accumulation decreased to 74.53%, 61.54%, and 47.86% after incubation with 5 mu M, 10 mu M, and 20 mu M pelargonidin, respectively, when compared with DMSO group. Furthermore, pelargonidin treatment led to decrease in glucose consumption. Western blot assay illustrated that the expression of PPAR-gamma was suppressed to 63.25%, 47.52%, and 21.23% after incubation with 5 mu M, 10 mu M, and 20 mu M pelargonidin when compared with DMSO group. Then, we measured the expression of some target proteins of PPAR-gamma, and found that pelargonidin decreased the expressions of HMGCR, LPL, Glut4, and A-FABP. Besides, the result of Luciferase Reporter Assay indicated that pelargonidin inhibited PPAR-gamma transcription activity. These results indicated that pelargonidin exerts anti-adipogenic activity in 3T3-L1 cells through inhibition of PPAR-gamma signaling pathway, and pelargonidin could be used as a potential anti-obesity agent.