A Gain-of-Function Mutant of IAA15 Inhibits Lateral Root Development by Transcriptional Repression ofLBDGenes in Arabidopsis

Abstract

Lateral root development is known to be regulated by Aux/IAA-ARF modules inArabidopsisthaliana. As components, several Aux/IAAs have participated in these Aux/IAA-ARF modules. In this study, to identify the biological function of IAA15 in plant developments, transgenic plant overexpressing the gain-of-function mutant of IAA15 (IAA15(P75S)OX) under the control of dexamethasone (DEX) inducible promoter, in which IAA15 protein was mutated by changing Pro-75 residue to Ser at the degron motif in conserved domain II, was constructed. As a result, we found that IAA15(P75S)OX plants show a decreased number of lateral roots. Coincidently,IAA15promoter-GUS reporter analysis revealed thatIAA15transcripts were highly detected in all stages of developing lateral root tissues. It was also verified that the IAA15(P75S)protein is strongly stabilized against proteasome-mediated protein degradation by inhibiting its poly-ubiquitination, resulting in the transcriptional repression of auxin-responsive genes. In particular, transcript levels ofLBD16andLBD29, which are positive regulators of lateral root formation, dramatically repressed in IAA15(P75S)OX plants. Furthermore, it was elucidated that IAA15 interacts with ARF7 and ARF19 and binds to the promoters ofLBD16andLBD29, strongly suggesting that IAA15 represses lateral root formation through the transcriptional suppression ofLBD16andLBD29by inhibiting ARF7 and ARF19 activity. Taken together, this study suggests that IAA15 also plays a key negative role in lateral root formation as a component of Aux/IAA-ARF modules.