Cellular Function of Annexin A1 Protein Mimetic Peptide Ac2-26 in Human Skin Keratinocytes HaCaT and Fibroblast Detroit 551 Cells
DC Field | Value | Language |
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dc.contributor.author | Kim, Seong Min | - |
dc.contributor.author | Ha, Sang Eun | - |
dc.contributor.author | Vetrivel, Preethi | - |
dc.contributor.author | Kim, Hun Hwan | - |
dc.contributor.author | Bhosale, Pritam Bhagwan | - |
dc.contributor.author | Park, Jung Eun | - |
dc.contributor.author | Heo, Jeong Doo | - |
dc.contributor.author | Kim, Young Sil | - |
dc.contributor.author | Kim, Gon Sup | - |
dc.date.accessioned | 2022-12-26T12:16:43Z | - |
dc.date.available | 2022-12-26T12:16:43Z | - |
dc.date.issued | 2020-11 | - |
dc.identifier.issn | 2072-6643 | - |
dc.identifier.issn | 2072-6643 | - |
dc.identifier.uri | https://scholarworks.gnu.ac.kr/handle/sw.gnu/5980 | - |
dc.description.abstract | Inflammation of the skin is the most common dermatological problem in human. The anti-inflammatory mediated responses of the skin cells provide a mechanism for combating these conditions. Annexin A1 (AnxA1) is one of the proteins that has been shown to have a potent anti-inflammatory effect. However, the effects and mechanisms of AnxA1 in skin keratinocyte and fibroblast have not been reported yet. In the current study, we hypothesized that Ac2-26, AnxA1 mimetic peptide, ameliorates inflammation and wrinkle formation in human skin cells. Therefore, we aimed to identify whether Ac2-26 has anti-inflammatory and anti-wrinkle effects in human keratinocyte (HaCaT) and fibroblast (Detroit 551) cells, respectively. Human HaCaT cells were stimulated by TNF-alpha/IFN-gamma with or without Ac2-26, to identify the anti-inflammatory effect. Human Detroit 551 cells were treated with Ac2-26 to verify the anti-wrinkle effect. Initially, cell cytotoxicity was carried out in each cell line treated using Ac2-26 by MTT assay. Human MDA, IL-8, and procollagen secretion were detected by ELISA assay. The inflammatory chemokines were measured by qRT-PCR analysis. To demonstrate the mechanism, MAPK, NF-kappa B, JAK/STAT, and MMPs were analyzed by Western blotting. As a result, we identified that Ac2-26 significantly decreased the expression of TNF-alpha/IFN-gamma-stimulated pro-inflammatory chemokines, including IL-1 beta, IL-6, IL-8, MDC, TARC, and TNF-alpha, by inhibiting the activation of MAPK, NF-kappa B, and JAK/STAT pathway in TNF-alpha/IFN-gamma-stimulated HaCaT human keratinocytes. In addition, we also identified that Ac2-26 significantly induced collagen synthesis by generating pro-collagen, and suppressed collagen degradation by inhibiting the collagenase MMP-1 and MMP-8 expression. Collectively, these results suggest that Ac2-26 shows anti-inflammatory and anti-wrinkling effect. These effects may lead to the development of preventive and therapeutic application for inflammation-related skin disease and wrinkle formation. | - |
dc.format.extent | 12 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | MDPI | - |
dc.title | Cellular Function of Annexin A1 Protein Mimetic Peptide Ac2-26 in Human Skin Keratinocytes HaCaT and Fibroblast Detroit 551 Cells | - |
dc.type | Article | - |
dc.publisher.location | 스위스 | - |
dc.identifier.doi | 10.3390/nu12113261 | - |
dc.identifier.scopusid | 2-s2.0-85094106164 | - |
dc.identifier.wosid | 000593691800001 | - |
dc.identifier.bibliographicCitation | NUTRIENTS, v.12, no.11, pp 1 - 12 | - |
dc.citation.title | NUTRIENTS | - |
dc.citation.volume | 12 | - |
dc.citation.number | 11 | - |
dc.citation.startPage | 1 | - |
dc.citation.endPage | 12 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Nutrition & Dietetics | - |
dc.relation.journalWebOfScienceCategory | Nutrition & Dietetics | - |
dc.subject.keywordPlus | NF-KAPPA-B | - |
dc.subject.keywordPlus | INFLAMMATORY CYTOKINES | - |
dc.subject.keywordPlus | ANTI-WRINKLE | - |
dc.subject.keywordPlus | P38 MAPK | - |
dc.subject.keywordPlus | TNF | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | BLOCKADE | - |
dc.subject.keywordPlus | DISEASES | - |
dc.subject.keywordPlus | EXTRACT | - |
dc.subject.keywordAuthor | annexin A1 | - |
dc.subject.keywordAuthor | Ac2-26 | - |
dc.subject.keywordAuthor | anti-inflammation | - |
dc.subject.keywordAuthor | anti-wrinkle | - |
dc.subject.keywordAuthor | skin disease | - |
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