Aquariibacter albus gen. nov., sp. nov., a new member of the order Burkholderiales, isolated from a freshwater aquarium

Abstract

A novel bacterium, strain SJAQ100(T), was isolated from a freshwater aquarium and was characterized taxonomically and phylogenetically. Strain SJAQ100(T) was a Gram-stain-negative, aerobic, rod-shaped and non-motile bacterium. The strain grew optimally with 0 % NaCl and at 25-37 degrees C on Reasoner's 2A agar. Phylogenetic analysis based on the 16S rRNA gene sequences revealed that the strain SJAQ100(T) clustered with members of Burkholderiales incertae sedis in the order Burkholderiales, but sequence similarities to known species were less than 96.5 %. The genomic DNA G+C content of strain SJAQ100(T) was 71.2 mol%. Genomic comparisons of strain SJAQ100(T) with species in the order Burkholderiales were made using the Genome-to-Genome Distance Calculator, average nucleotide identity and average amino acid identity analyses (values indicated <= 22.1, <= 78.1, and <= 68.1 % respectively). Strain SJAQ100(T) contained C-16:0 and C16:1 omega 7c/C-16:1 omega 6c as major fatty acids and Q-8 as the major quinone. The major polyamines were putrescine and cadaverine. Strain SJAQ100(T) contained phosphatidylethanolamine and diphosphatidylglycerol as major polar lipids. Based on the genotypic, chemotaxonomic and phenotypic results, strain SJAQ100(T) represents a novel genus and species, Aquariibacter albus gen. nov., sp. nov., which belongs to order Burkholderiales and the class Betaproteobacteria. The type strain is SJAQ100(T) (=KCTC 72203(T)=CGMCC 1.18869(T)=MCC 4385(T)).