Protection of palmitic acid treatment in RAW264.7 cells and BALB/c mice during Brucella abortus 544 infection
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Reyes, Alisha Wehdnesday Bernardo | - |
dc.contributor.author | Tran Xuan Ngoc Huy | - |
dc.contributor.author | Son Hai Vu | - |
dc.contributor.author | Kim, Hyun Jin | - |
dc.contributor.author | Lee, Jin Ju | - |
dc.contributor.author | Choi, Jeong Soo | - |
dc.contributor.author | Lee, John Hwa | - |
dc.contributor.author | Kim, Suk | - |
dc.date.accessioned | 2022-12-26T10:31:21Z | - |
dc.date.available | 2022-12-26T10:31:21Z | - |
dc.date.created | 2022-12-12 | - |
dc.date.issued | 2021-03 | - |
dc.identifier.issn | 1229-845X | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gnu/handle/sw.gnu/3994 | - |
dc.description.abstract | Background: We previously elucidated the protective mechanism of Korean red ginseng oil (RGO) against Brucella abortus infection, and our phytochemical analysis revealed that palmitic acid (PA) was an abundant component of RGO. Consequently, we investigated the contribution of PA against B. abortus. Objectives: We aimed to investigate the efficacy of PA against B. abortus infection using a murine cell line and a murine model. Methods: Cell viability, bactericidal, internalization, and intracellular replication, western blot, nitric oxide (NO), and superoxide (O-2(-)) analyses and flow cytometry were performed to determine the effects of PA on the progression of B. abortus infection in macrophages. Flow cytometry for cytokine analysis of serum samples and bacterial counts from the spleens were performed to determine the effect of PA in a mouse model. Results: PA did not affect the growth of B. abortus. PA treatment in macrophages did not change B. abortus uptake but it did attenuate the intracellular survivability of B. abortus. Incubation of cells with PA resulted in a modest increase in sirtuin 1 (SIRT1) expression. Compared to control cells, reduced nitrite accumulation, augmented O-2(-), and enhanced pro-inflammatory cytokine production were observed in PA-treated B. abortus-infected cells. Mice orally treated with PA displayed a decreased serum interleukin-10 level and enhanced bacterial resistance. Conclusions: Our results suggest that PA participates in the control of B. abortus within murine macrophages, and the in vivo study results confirm its efficacy against the infection. However, further investigations are encouraged to completely characterize the mechanisms involved in the inhibition of B. abortus infection by fatty acids. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | KOREAN SOC VETERINARY SCIENCE | - |
dc.subject | TOLL-LIKE RECEPTOR-4 | - |
dc.subject | CYTOKINE PRODUCTION | - |
dc.subject | FATTY-ACIDS | - |
dc.subject | SUSCEPTIBILITY | - |
dc.subject | MACROPHAGES | - |
dc.subject | ADIPOCYTES | - |
dc.subject | RESISTANCE | - |
dc.subject | SURVIVAL | - |
dc.title | Protection of palmitic acid treatment in RAW264.7 cells and BALB/c mice during Brucella abortus 544 infection | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Hyun Jin | - |
dc.contributor.affiliatedAuthor | Kim, Suk | - |
dc.identifier.doi | 10.4142/jvs.2021.22.e18 | - |
dc.identifier.scopusid | 2-s2.0-85102812891 | - |
dc.identifier.wosid | 000635370300004 | - |
dc.identifier.bibliographicCitation | JOURNAL OF VETERINARY SCIENCE, v.22, no.2 | - |
dc.relation.isPartOf | JOURNAL OF VETERINARY SCIENCE | - |
dc.citation.title | JOURNAL OF VETERINARY SCIENCE | - |
dc.citation.volume | 22 | - |
dc.citation.number | 2 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART002696480 | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Veterinary Sciences | - |
dc.relation.journalWebOfScienceCategory | Veterinary Sciences | - |
dc.subject.keywordPlus | TOLL-LIKE RECEPTOR-4 | - |
dc.subject.keywordPlus | CYTOKINE PRODUCTION | - |
dc.subject.keywordPlus | FATTY-ACIDS | - |
dc.subject.keywordPlus | SUSCEPTIBILITY | - |
dc.subject.keywordPlus | MACROPHAGES | - |
dc.subject.keywordPlus | ADIPOCYTES | - |
dc.subject.keywordPlus | RESISTANCE | - |
dc.subject.keywordPlus | SURVIVAL | - |
dc.subject.keywordAuthor | B. abortus | - |
dc.subject.keywordAuthor | macrophages | - |
dc.subject.keywordAuthor | palmitic acid | - |
dc.subject.keywordAuthor | spleen | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
Gyeongsang National University Central Library, 501, Jinju-daero, Jinju-si, Gyeongsangnam-do, 52828, Republic of Korea+82-55-772-0533
COPYRIGHT 2022 GYEONGSANG NATIONAL UNIVERSITY LIBRARY. ALL RIGHTS RESERVED.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.