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Cited 3 time in webofscience Cited 4 time in scopus
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Identification of regulated proteins by resveratrol in glutamate-induced cortical injury of newborn ratsopen access

Authors
Gim, Sang-APark, Dong-JuKang, Ju-BinShah, Fawad-AliKoh, Phil-Ok
Issue Date
Apr-2021
Publisher
Maruzen Co., Ltd/Maruzen Kabushikikaisha
Keywords
cerebral cortex; glutamate; neonate; proteomics; resveratrol
Citation
Journal of Veterinary Medical Science, v.83, no.4, pp 724 - 733
Pages
10
Indexed
SCIE
SCOPUS
Journal Title
Journal of Veterinary Medical Science
Volume
83
Number
4
Start Page
724
End Page
733
URI
https://scholarworks.gnu.ac.kr/handle/sw.gnu/3904
DOI
10.1292/jvms.21-0013
ISSN
0916-7250
1347-7439
Abstract
Glutamate induces neuronal damage by generating oxidative stress and neurotoxicities. The neurological damage caused by glutamate is more severe during brain development in newborns than in adults. Resveratrol is naturally present in a variety of fruits and medicinal plants and exerts a neuroprotective effect against brain damage. The goal of this study was to evaluate the neuroprotective effects of resveratrol and to identify changed proteins in response to resveratrol treatment during glutamate-induced neonatal cortical damage. Sprague-Dawley rat pups (7 days old) were randomly divided into vehicle, resveratrol, glutamate, and glutamate and resveratrol groups. The animals were intraperitoneally injected with glutamate (10 mg/kg) and/or resveratrol (20 mg/kg) and their brain tissue was collected 4 hr after drug administration. Glutamate exposure caused severe histopathological changes, while resveratrol attenuated this damage. We identified regulated proteins by resveratrol in glutamate-induced cortical damaged tissue using two-dimensional gel electrophoresis and mass spectrometry. Among identified proteins, we focused on eukaryotic initiation factor 4A2, gamma-enolase, protein phosphatase 2A subunit B, and isocitrate dehydrogenase. These proteins decreased in the glutamate-treated group, whereas the combination treatment of glutamate and resveratrol attenuated these protein reductions. These proteins are anti-oxidant proteins and anti-apoptotic proteins. These results suggest that glutamate induces brain cortical damage in newborns; resveratrol exerts a neuroprotective effect by controlling expression of various proteins with anti- oxidant and anti-apoptotic functions.
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