Constitutive Expression of TERT Enhances beta-Klotho Expression and Improves Age-Related Deterioration in Early Bovine Embryos
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Xu, Lianguang | - |
dc.contributor.author | Idrees, Muhammad | - |
dc.contributor.author | Joo, Myeong-Don | - |
dc.contributor.author | Sidrat, Tabinda | - |
dc.contributor.author | Wei, Yiran | - |
dc.contributor.author | Song, Seok-Hwan | - |
dc.contributor.author | Lee, Kyeong-Lim | - |
dc.contributor.author | Kong, Il-Keun | - |
dc.date.accessioned | 2022-12-26T10:30:32Z | - |
dc.date.available | 2022-12-26T10:30:32Z | - |
dc.date.created | 2022-12-12 | - |
dc.date.issued | 2021-05 | - |
dc.identifier.issn | 1661-6596 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gnu/handle/sw.gnu/3781 | - |
dc.description.abstract | Age-associated decline in oocyte quality is one of the dominant factors of low fertility. Aging alters several key processes, such as telomere lengthening, cell senescence, and cellular longevity of granulosa cells surrounding oocyte. To investigate the age-dependent molecular changes, we examined the expression, localization, and correlation of telomerase reverse transcriptase (TERT) and beta-Klotho (KLB) in bovine granulosa cells, oocytes, and early embryos during the aging process. Herein, cumulus-oocyte complexes (COCs) obtained from aged cows (>120 months) via ovum pick-up (OPU) showed reduced expression of beta-Klotho and its co-receptor fibroblast growth factor receptor 1 (FGFR1). TERT plasmid injection into pronuclear zygotes not only markedly enhanced day-8 blastocysts' development competence (39.1 +/- 0.8%) compared to the control (31.1 +/- 0.5%) and D-galactose (17.9 +/- 1.0%) treatment groups but also enhanced KLB and FGFR1 expression. In addition, plasmid-injected zygotes displayed a considerable enhancement in blastocyst quality and implantation potential. Cycloastragenol (CAG), an extract of saponins, stimulates telomerase enzymes and enhances KLB expression and alleviates age-related deterioration in cultured primary bovine granulosa cells. In conclusion, telomerase activation or constitutive expression will increase KLB expression and activate the FGFR1/beta-Klotho pathway in bovine granulosa cells and early embryos, inhibiting age-related malfunctioning. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | MDPI | - |
dc.subject | DEVELOPMENTAL COMPETENCE | - |
dc.subject | MATERNAL AGE | - |
dc.subject | IN-VITRO | - |
dc.subject | TELOMERASE | - |
dc.subject | INFLAMMATION | - |
dc.subject | INJURY | - |
dc.subject | ROLES | - |
dc.subject | CELLS | - |
dc.subject | GENE | - |
dc.title | Constitutive Expression of TERT Enhances beta-Klotho Expression and Improves Age-Related Deterioration in Early Bovine Embryos | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kong, Il-Keun | - |
dc.identifier.doi | 10.3390/ijms22105327 | - |
dc.identifier.scopusid | 2-s2.0-85105880535 | - |
dc.identifier.wosid | 000661987700001 | - |
dc.identifier.bibliographicCitation | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v.22, no.10 | - |
dc.relation.isPartOf | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | - |
dc.citation.title | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | - |
dc.citation.volume | 22 | - |
dc.citation.number | 10 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.subject.keywordPlus | DEVELOPMENTAL COMPETENCE | - |
dc.subject.keywordPlus | MATERNAL AGE | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.subject.keywordPlus | TELOMERASE | - |
dc.subject.keywordPlus | INFLAMMATION | - |
dc.subject.keywordPlus | INJURY | - |
dc.subject.keywordPlus | ROLES | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | GENE | - |
dc.subject.keywordAuthor | beta-Klotho | - |
dc.subject.keywordAuthor | FGFR1 | - |
dc.subject.keywordAuthor | TERT | - |
dc.subject.keywordAuthor | cycloastragenol | - |
dc.subject.keywordAuthor | bovine embryo | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
Gyeongsang National University Central Library, 501, Jinju-daero, Jinju-si, Gyeongsangnam-do, 52828, Republic of Korea+82-55-772-0533
COPYRIGHT 2022 GYEONGSANG NATIONAL UNIVERSITY LIBRARY. ALL RIGHTS RESERVED.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.